Application
Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody its detection by antibody IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of alpha tubulin is around 50 kDa. L = lysate IPr = immunoprecipitate (reducing conditions) IPn = immunoprecipitate (non-reducing conditions)| Product Name | alpha Tubulin antibody [TU-16] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [TU - 16] to alpha TubulinTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin. |
| Immunogen | Full length native protein (purified) (Pig). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | TU-16 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-PIPWB |
| ICC: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMousePigRatHamsterBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TUBA1 Tubulin alpha 1 chain Tubulin alpha |
|---|---|
| Target | alpha Tubulin |
| Tissue Specificity | The antibody binds tubulin under denaturing and non-denaturing conditions. |
| Cellular Localization | Major constituent of microtubules. |
Application
Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody its detection by antibody IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of alpha tubulin is around 50 kDa. L = lysate IPr = immunoprecipitate (reducing conditions) IPn = immunoprecipitate (non-reducing conditions)| Application Notes | ICC: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4 °C for 3 months. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
