Application
Western blot analysis of ATP12A Antibody (Center) in K562 cell line lysates (35ug/lane). ATP12A (arrow) was detected using the purified Pab.Application
ATP12A Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human skin reacted with ATP12A Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ATP12A antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ATP12A (Internal)The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 839~868 amino acids from the Center region of human ATP12A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Potassiumtransporting ATPase alpha chain2 ATP1AL1 Potassiumtransporting ATPase alpha chain 2 Non-gastric H(+)/K(+) ATPase subunit alpha Nongastric H(+)/K(+) ATPase subunit alpha Potassium-transporting ATPase alpha chain 2 ATP12A Proton pump |
|---|---|
| Target | ATP12A |
| Molecular Weight(MW) | 115.511 kDa (note) |
Application
Western blot analysis of ATP12A Antibody (Center) in K562 cell line lysates (35ug/lane). ATP12A (arrow) was detected using the purified Pab.
Application
ATP12A Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human skin reacted with ATP12A Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
