Application
Western blot analysis of extracts of various cell lines, using ATP5A1 antibody.Application
Immunofluorescence analysis of U20S cell using ATP5A1 antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded human esophageal cancer using ATP5A1 antibody at dilution of 1:100 (x40 lens).Application
Immunohistochemistry of paraffin-embedded human liver injury using ATP5A1 antibody at dilution of 1:100 (x40 lens).| Product Name | ATP5A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP5A1This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012] |
| Immunogen | Recombinant protein of human ATP5A1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATP5A ATP5AL2 OMR ATPM ORM MOM2 hATP1 ATP synthase H+ transporting mitochondrial F1 complex alpha subunit 1 cardiac muscle |
|---|---|
| Target | ATP5A1 |
Application
Western blot analysis of extracts of various cell lines, using ATP5A1 antibody.
Application
Immunofluorescence analysis of U20S cell using ATP5A1 antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded human esophageal cancer using ATP5A1 antibody at dilution of 1:100 (x40 lens).
Application
Immunohistochemistry of paraffin-embedded human liver injury using ATP5A1 antibody at dilution of 1:100 (x40 lens).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
