Application
Immunoprecipitation of ATR protein from HCT-116 whole cell extracts treated with 2 mM Hydroxyurea 6 hrs using 5 μg of ATR antibody or ATR antibody . Western blot analysis was performed using ATR antibody . EasyBlot anti-Mouse IgG was used as a secondary reagent.Application
Untreated (–) and treated (+) HCT116 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [GT222] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.| Product Name | ATR (phospho Thr1989) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT222] to ATR (phospho Thr1989) (ataxia telangiectasia and Rad3 related)The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATR (phospho Thr1989). The exact sequence is proprietary. |
| Modification | p-Thr1989 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT222 |
| Host Species | Mouse |
| Tested Applications | IP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ataxia telangiectasia and Rad3 related SCKL1 MEC1 ATR SCKL FRP1 FCTCS |
|---|---|
| Target | ATR (phospho Thr1989) |
| Molecular Weight(MW) | 301 kDa (note) |
| Cellular Localization | Nucleus |
Application
Immunoprecipitation of ATR protein from HCT-116 whole cell extracts treated with 2 mM Hydroxyurea 6 hrs using 5 μg of ATR antibody or ATR antibody . Western blot analysis was performed using ATR antibody . EasyBlot anti-Mouse IgG was used as a secondary reagent.
Application
Untreated (–) and treated (+) HCT116 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [GT222] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
