Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Detection of ATM (Anti-ATM 2C1, ) or ATR (Anti-ATR 2B5,) by western blot in human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of HeLa, using ATR antibody at 1:100 dilution.Application
Raji whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | ATR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2B5] to ATRAtaxia Telangiectasia Mutated (ATM) and Rad3-related (ATR) protein is a member of the phosphatidyl-inositol 3-kinase (PI 3-kinase) like family of protein kinases. ATR has a predicted molecular mass of approximately 301 kDa, and it has been shown to be involved in cellular responses to DNA damage. Specifically, it plays a role in checkpoint activation in response to either the presence of stalled replication forks, or by the presence of lesions caused by UV light. Inactivation of ATR is an embryonic lethal event in mice. |
| Immunogen | Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 2B5 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWBFunctional Assay |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | SCKL1 Q13535 ATR 210600 MEC1 FRP1 SCKL 545 |
|---|---|
| Target | ATR |
| Molecular Weight(MW) | 317 kDa (note) |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Detection of ATM (Anti-ATM 2C1, ) or ATR (Anti-ATR 2B5,) by western blot in human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of HeLa, using ATR antibody at 1:100 dilution.
Application
Raji whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | Raji , Raji nuclear extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH7.2 |
