Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using BCL10 antibody at 1:250 dilution.Application
BCL10 antibody detects BCL10 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 12 % SDS-PAGE BCL10 antibody dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE diluted at 1:1000| Product Name | BCL10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BCL10 (B-cell CLL/lymphoma 10)This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BCL10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rhesus Monkey, BovineRat (96%), Pig (96%), Rhesus Monkey (99%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CARMEN antibody CIPER antibody CLAP antibody c-E10 antibody mE10 antibody BCL10 antibody B-cell lymphoma/leukemia 10 antibody cellular-E10 antibody cCARMEN antibody CARD containing molecule enhancing NF-kB antibody hCLAP antibody CED-3/ICH-1 pro |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) (2) |
| Cellular Localization | Cytoplasm , perinuclear region |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using BCL10 antibody at 1:250 dilution.
Application
BCL10 antibody detects BCL10 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 12 % SDS-PAGE BCL10 antibody dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE diluted at 1:1000| Positive Control | Jurkat , Neuro2A , GL261 , C8D30 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
