Application
BCL10 antibody [N1C3] detects BCL10 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. BCL10 antibody [N1C3] diluted at 1:500.Application
BCL10 antibody [N1C3] immunoprecipitates BCL10 protein in IP experiments. IP samples: Raji whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of BCL10 protein by 4 μg BCL10 antibody [N1C3] 12 % SDS-PAGE The immunoprecipitated BCL10 protein was detected by BCL10 antibody [N1C3] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Sample(30 ug whole cell lysate) A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE BCL10 antibody diluted at 1:1000Application
BCL10 antibody [N1C3] detects BCL10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. BCL10 antibody [N1C3] diluted at 1:500.Application
Cross-linked ChIP was performed with MCF-7 chromatin extract treated with TNF-a (10 ng/ml for 30 min) and 5 μg of either control rabbit IgG or anti-BCL10 antibody. The precipitated DNA was detected by PCR with primer set targeting to IκBα.| Product Name | BCL10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BCL10 (B-cell CLL/lymphoma 10)This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq] |
| Immunogen | Full length human BCL10 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rhesus Monkey, BovineRat (91%), Pig (92%), Rhesus Monkey (99%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CARMEN antibody CIPER antibody CLAP antibody c-E10 antibody mE10 antibody BCL10 antibody B-cell lymphoma/leukemia 10 antibody cellular-E10 antibody cCARMEN antibody CARD containing molecule enhancing NF-kB antibody hCLAP antibody CED-3/ICH-1 pro |
|---|---|
| Target | BCL10 |
| Molecular Weight(MW) | 26 kDa (note) (2) |
| Cellular Localization | Cytoplasm , perinuclear region |
Application
BCL10 antibody [N1C3] detects BCL10 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. BCL10 antibody [N1C3] diluted at 1:500.
Application
BCL10 antibody [N1C3] immunoprecipitates BCL10 protein in IP experiments. IP samples: Raji whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of BCL10 protein by 4 μg BCL10 antibody [N1C3] 12 % SDS-PAGE The immunoprecipitated BCL10 protein was detected by BCL10 antibody [N1C3] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Sample(30 ug whole cell lysate) A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE BCL10 antibody diluted at 1:1000
Application
BCL10 antibody [N1C3] detects BCL10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. BCL10 antibody [N1C3] diluted at 1:500.
Application
Cross-linked ChIP was performed with MCF-7 chromatin extract treated with TNF-a (10 ng/ml for 30 min) and 5 μg of either control rabbit IgG or anti-BCL10 antibody. The precipitated DNA was detected by PCR with primer set targeting to IκBα.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
