| Product Name | Bcl2L2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [90316.11] to BCL 2L2Bcl 2L2 or Bcl-w is a member of the Bcl 2 family of proteins that regulates outer mitochondrial membrane permeability. At least fifteen Bcl 2 family members have been identified in mammalian cells. Pro-survival members of the Bcl 2 family, which include Bcl XL, Bcl X, Mcl 1 and A1, inhibit apoptosis in response to a wide variety of cytotoxic insults, whereas the proapoptotic family members (e.g. Bax, Bak, Bad, Bik and Bid) in general antagonize the function of the prosurvival family members. Bcl w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. It is required for normal sperm maturation. Natural Bcl w contains a carboxylterminal mitochondria targeting sequence. Recombinant Bcl w lacks the mitochondrial targeting sequence but maintains its ability to neutralize pro-apoptotic Bcl 2 family members. Neutralization by Bcl w appears to occur through binding the BH3 region of pro-apoptotic Bcl 2 family members. This activity does not require the mitochondrial targeting sequence. |
| Immunogen | Purified recombinant human Bcl-w (amino acids 1-172, accession number U59747) containing six histidines at the carboxy-terminus as immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 90316.11 |
| Host Species | Mouse |
| Tested Applications | Dot |
| Dot: For immunoblotting, a working antibody concentration of approximately 1.0 μg/ml is recommended using recombinant human Bcl-w (1 ng) and extracts from 1 x 105 human WS1 (embryonic skin) fibroblasts and 2 x 105 mouse CTLL2 (cytoxic T cell line) cells in an enhanced chemiluminescent (ECL) assay. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | KIAA0271 601931 BCL2L2 BCLW 599 Q92843 BCL 2L2 BCL-W BCL W |
|---|---|
| Target | BCL 2L2 |
| Cellular Localization | Cytoplasmic |
| Application Notes | Dot: For immunoblotting, a working antibody concentration of approximately 1.0 μg/ml is recommended using recombinant human Bcl-w (1 ng) and extracts from 1 x 105 human WS1 (embryonic skin) fibroblasts and 2 x 105 mouse CTLL2 (cytoxic T cell line) cells in an enhanced chemiluminescent (ECL) assay. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees C. For prolonged storage, freeze in working aliquots at -20 degrees C. Repeated freezing and thawing is not recommeneded. Do not store in a frost-free freezer. |
| Storage Buffer | Preservative: 0.05% Sodium Azide. Constituents: PBS |
