Application
WB analysis of Bid expression in Jurkat cell lysate.| Product Name | Bid antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to BidBid, a BH3 domain-containing pro-apoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent pro-apoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas. |
| Immunogen | Synthetic peptide, surrounding amino acid 37 of Human Bid. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIPWB |
| IHC-Fr: Use at a concentration of 20 - 40 μg/ml. IP: Use at a concentration of 5 - 10 μg/ml. WB: Use at a concentration of 1 μg/ml. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bid, 637, MGC42355, 601997, P55957, MGC15319 |
|---|---|
| Target | Bid |
| Tissue Specificity | This antibody detects both full length (22 kDa) and cleaved fragments (15 kDa) of Bid. |
| Cellular Localization | Cytoplasmic |
Application
WB analysis of Bid expression in Jurkat cell lysate.| Application Notes | IHC-Fr: Use at a concentration of 20 - 40 μg/ml. IP: Use at a concentration of 5 - 10 μg/ml. WB: Use at a concentration of 1 μg/ml. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 1 % BSA, 0.02% thimerosal and 50% glycerol |
