| Product Name | Bid antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Bid Cleavage SiteBH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery. |
| Immunogen | Synthetic peptide (Mouse)corresponding to N-terminus of cleavage site (59/60). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.5 - 1.0 μg/ml. Predicted molecular weight: 15 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bid, 637, MGC42355, 601997, P55957, MGC15319 |
|---|---|
| Target | Bid |
| Tissue Specificity | Data show that this product only recognizes the cleaved mouse BID and not full length. |
| Cellular Localization | Cytoplasmic in its inactive form and translocates onto mitochondria once cleaved. |
| Positive Control | TNF alpha treated L929 cells |
|---|---|
| Application Notes | WB: Use at a concentration of 0.5 - 1.0 μg/ml. Predicted molecular weight: 15 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 1% BSA, PBS. pH 7.2 |
