Application
WB analysis of Bid expression in 3T3 cell lysate.| Product Name | Bid antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to BidBid, a BH3 domain-containing pro-apoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent pro-apoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas. |
| Immunogen | Synthetic peptide surrounding amino acid 45 of Mouse Bid. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP: Use at a concentration of 20 μg/ml.WB:Use at a concentration of 0.5-5 µg/ml. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Mouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bid, 637, MGC42355, 601997, P55957, MGC15319 |
|---|---|
| Target | Bid |
| Tissue Specificity | This antibody detects both full length (22 kDa) and cleaved fragment (15 kDa) of mouse Bid. |
| Cellular Localization | Cytoplasmic |
Application
WB analysis of Bid expression in 3T3 cell lysate.| Application Notes | IP: Use at a concentration of 20 μg/ml.WB:Use at a concentration of 0.5-5 µg/ml. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% thimerosal |
