Application
BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
BRCA1 antibody 6B4 and BRCA1 antibody 17F8 were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.Application
BRCA1 antibody 6B4 and BRCA1 antibody 17F8 was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | BRCA1 antibody [17F8] - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [17F8] to BRCA1BRCA1-17F8 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the Aexon 11 splice variant. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation. |
| Immunogen | Protein fragment expressed in E. coli corresponding to amino acids 762-1315. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 17F8 |
| Host Species | Mouse |
| Tested Applications | ELISA,ICC/IF,IHC,IHC-P,IP,WB,ChIP |
| For ICC/IF: Use at a concentration of 1 µg/ml. For WB: 1:500-1:3000. For IHC-P: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher | |
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | 113705, RNF53, BRCA1, BRCAI, 672, PSCP, BRCC1, P38398, IRIS, BROVCA1, PNCA4 |
|---|---|
| Target | BRCA1 |
| Molecular Weight(MW) | 220 kDa (note) |
| Tissue Specificity | This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. |
| Cellular Localization | Nuclear/Cytoplasmic |
| Light Chain Type | kappa |
Application
BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
BRCA1 antibody 6B4 and BRCA1 antibody 17F8 were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.
Application
BRCA1 antibody 6B4 and BRCA1 antibody 17F8 was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Application Notes | For ICC/IF: Use at a concentration of 1 µg/ml. For WB: 1:500-1:3000. For IHC-P: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH7.2 containing no preservatives |
