Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRCA1 antibody at 1:100 dilution.| Product Name | BRCA1 antibody [9F12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [9F12] to BRCA1BRCA1-9F12 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation. |
| Immunogen | BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 9F12 |
| Host Species | Mouse |
| Tested Applications | ICC/IF,IP,WB |
| For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 1-10 µg/ml. Predicated molecular weight: 208 kDa. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | 113705, RNF53, BRCA1, BRCAI, 672, PSCP, BRCC1, P38398, IRIS, BROVCA1, PNCA4 |
|---|---|
| Target | BRCA1 |
| Molecular Weight(MW) | 220 kDa (note) |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRCA1 antibody at 1:100 dilution.| Application Notes | For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 1-10 µg/ml. Predicated molecular weight: 208 kDa. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles |
| Storage Buffer | Phosphate-buffered saline, pH7.2, no preservative added |
