Application
Immunohistochemical analysis of paraffin-embedded Breast ca, using BRCA1 antibody at 1:200 dilution.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | BRCA1 antibody [8F7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [8F7] to BRCA1BRCA1-8F7 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation. |
| Immunogen | Protein fragment expressed in E. coli corresponding to amino acids 341-748. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8F7 |
| Host Species | Mouse |
| Tested Applications | IHC,IHC-P,IP,WB |
| For IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. For IP: Use at an assay dependent dilution. For WB: 1:500-1:3000. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | 113705, RNF53, BRCA1, BRCAI, 672, PSCP, BRCC1, P38398, IRIS, BROVCA1, PNCA4 |
|---|---|
| Target | BRCA1 |
| Molecular Weight(MW) | 220 kDa (note) |
| Tissue Specificity | This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. |
| Cellular Localization | Nuclear/Cytoplasmic |
| Light Chain Type | kappa |
Application
Immunohistochemical analysis of paraffin-embedded Breast ca, using BRCA1 antibody at 1:200 dilution.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Application Notes | For IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. For IP: Use at an assay dependent dilution. For WB: 1:500-1:3000. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing no preservatives |
