Application
Calcineurin A antibody detects Calcineurin A protein by western blot analysis. Non-transfected (-) and Calcineurin A-transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Calcineurin A antibody detects Calcineurin A protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Calcineurin A antibody dilution: 1:500.Application
Calcineurin A antibody detects Calcineurin A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcineurin A protein stained by Calcineurin A antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.| Product Name | Calcineurin A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Calcineurin A (protein phosphatase 3, catalytic subunit, alpha isozyme) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calcineurin A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Dog, Pig, Rabbit, Rhesus Monkey, BovineZebrafish (93%), Xenopus laevis (98%), Dog (100%), Pig (99%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CALN antibody CALNA antibody CALNA1 antibody CCN1 antibody CNA1 antibody PPP2B antibody PPP3CA antibody calmodulin-dependent calcineurin A subunit alpha isoform antibody calcineurin A alpha antibody protein phosphatase 2B catalytic subunit alpha |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) (3) |
| Cellular Localization | Nucleus (By similarity) |
Application
Calcineurin A antibody detects Calcineurin A protein by western blot analysis. Non-transfected (-) and Calcineurin A-transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Calcineurin A antibody detects Calcineurin A protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Calcineurin A antibody dilution: 1:500.
Application
Calcineurin A antibody detects Calcineurin A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcineurin A protein stained by Calcineurin A antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.| Positive Control | human PPP3CA-transfected 293T , mouse brain , Rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
