| Product Name | Caldesmon antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CALD 8] to CaldesmonMonoclonal anti-Caldesmon may be used for the study of the involvement of caldesmon in contraction, cell movement, shape change, exocytosis and endocytosis. Caldesmon is an actin interacting and calmodulin binding protein found in smooth muscle and other cell types. Caldesmon occurs in both a high molecular weight (120-150 kDa) and a low molecular weight (71-80 kDa) form, depending on the tissue in which it is located. Caldesmon plays a major role in the regulation of smooth muscle and non-muscle contractile events. |
| Immunogen | Turkey gizzard caldesmon was used as the immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CALD 8 |
| Host Species | Mouse |
| Tested Applications | DIDICC/IFWB |
| DID: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. WB: Use at a dilution of 1/400 using a pig stomach caldesmon extract and immunoperoxidase labeling. By indirect immunofluorescence monoclonal anti-caldesmon stains frozen tissue sections from chicken, turkey, rabbit, guinea pig, and human tissue preparations. Caldesmon occurs in both a high molecular weight (120- 150 kDa) and a low molecular weight (71-80 kDa) form, depending on the tissue in which it is located. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanPigGuinea PigRabbitTurkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | L-CAD Q05682 LCAD CDM MGC21352 HCAD CALD1 Caldesmon 800 114213 NAG22 H-CAD L CAD H CAD |
|---|---|
| Target | Caldesmon |
| Tissue Specificity | The antibody localizes the high molecular weight form of caldesmon from pig stomach, chicken or turkey gizzard, and the low molecular weight form of caldesmon from chicken fibroblasts when used in an immunoblot technique. |
| Cellular Localization | Cytoplasmic |
| Application Notes | DID: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. WB: Use at a dilution of 1/400 using a pig stomach caldesmon extract and immunoperoxidase labeling. By indirect immunofluorescence monoclonal anti-caldesmon stains frozen tissue sections from chicken, turkey, rabbit, guinea pig, and human tissue preparations. Caldesmon occurs in both a high molecular weight (120- 150 kDa) and a low molecular weight (71-80 kDa) form, depending on the tissue in which it is located. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Raw Ascites |
