Application
Western blot analysis of Calpain 1 in mouse lung extract.Application
Immunofluorescent analysis of Calpain 1 in HeLa cells. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Calpain 1 antibody [9A4H8D3] at a dilution of 1:100 overnight at 4°C, washed with PBS and incubated with a proper secondary antibody. Calpain 1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.Application
Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with or without Calpain 1 antibody [9A4H8D3] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Product Name | Calpain 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [9A4H8D3] to Calpain 1 |
| Immunogen | Purified bovine skeletal muscle 80 kDa mu-calpain subunit. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 9A4H8D3 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-FIHC-PIPWB |
| ICC/IF: 1:100: |
|
| Species Reactivity | HumanMousePigRatYeastFishHamsterRabbitBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | CAPN1 calpain 1 (mu/I) large subunit |
|---|---|
| Target | Mu-Calpain |
| Tissue Specificity | This antibody does not cross-react with Calpain 2, n-calpain, calmodulin or calpastatin. |
Application
Western blot analysis of Calpain 1 in mouse lung extract.
Application
Immunofluorescent analysis of Calpain 1 in HeLa cells. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Calpain 1 antibody [9A4H8D3] at a dilution of 1:100 overnight at 4°C, washed with PBS and incubated with a proper secondary antibody. Calpain 1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Application
Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with or without Calpain 1 antibody [9A4H8D3] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Application Notes | ICC/IF: 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | Ascites containing 0.05 % sodium azide |
