Application
Western blot analysis for human Caspase-2 at 1:1000 on 20 ugs of HL60 whole cell lysate.| Product Name | Caspase 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [18E809] to Caspase 2Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 2 has a positive and a negative role in the regulation of the apoptotic process. Overexpression of the full caspase 2 (Nedd2/Ich1 ) mRNA in some cell types results in apoptosis, whereas overexpression of an alternative caspase 2 splice variant suppresses apoptosis induced by serum withdrawal. Caspase 2 may be activated in vitro by caspase 1, caspase 3 and the neutral serine protease granzyme B which is stored in the specialized lytic granules of cytotoxic lymphocytes. |
| Immunogen | The immunogen corresponding to a region within amino acids 400 and 450 of human Caspase 2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 18E809 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at a dilution of 1/500 - 1/1000. Predicted molecular weight: 48 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Caspase2 NEDD2 ICH1L/1S Caspase 2 ICH1L ICH1 835 ICH-1L/1S CASP2 P42575 CASP-2 600639 ICH-1L CASP 2 ICH 1L ICH 1L/1S |
|---|---|
| Target | Caspase 2 |
| Tissue Specificity | This antibody is useful for detecting human Caspase 2 long form, which is about 48 kD. |
Application
Western blot analysis for human Caspase-2 at 1:1000 on 20 ugs of HL60 whole cell lysate.| Positive Control | HL60 whole cell lysate. |
|---|---|
| Application Notes | WB: Use at a dilution of 1/500 - 1/1000. Predicted molecular weight: 48 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 0.2% Gelatin, PBS |
