Application
Western Blot: Caspase 8 Antibody (FLICE 4-1-20) - Detection of Caspase 8 (59 kDa) from HEK293 cell extract using (1:500). Lanes 1 and 2 contain inactive and active Caspase 8, respectively.| Product Name | Caspase 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal to human Caspase 8Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; inhibitors (activation by receptor cluster) and effetors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initaitor caspases, such as Caspase-8 and Caspase-9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse. |
| Immunogen | Full-length recombinant human Caspase 8 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | FLICE 4-1-20 |
| Host Species | Mouse |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:1,000 (ECL). May be used for Western analysis where a band is seen at 55 kDa representing the pro form of Caspase 8. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Q14790 MGC78473 MACH Caspase8 114550 CASP8 MCH5 Caspase 8 841 FLICE |
|---|---|
| Target | Caspase 8 |
| Tissue Specificity | Specific for Caspase 8. |
| Light Chain Type | kappa |
Application
Western Blot: Caspase 8 Antibody (FLICE 4-1-20) - Detection of Caspase 8 (59 kDa) from HEK293 cell extract using (1:500). Lanes 1 and 2 contain inactive and active Caspase 8, respectively.| Positive Control | Human kidney 293 cell lysates |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:1,000 (ECL). May be used for Western analysis where a band is seen at 55 kDa representing the pro form of Caspase 8. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid containing 0.1% sodium azide |
