Application
Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody.The lane on the right is treated with the antigen-specific peptide.Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | Caspase 9 (phospho Thr125) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caspase 9 (phospho Thr125)This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] |
| Immunogen | Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9. |
| Modification | p-Thr125 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC: 1:50 - 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | APAF3 APAF-3 ICE-LAP6 PPP1R56 caspase 9 apoptosis-related cysteine peptidase CASP9 MCH6 |
|---|---|
| Target | Caspase 9 (phospho Thr125) |
| Tissue Specificity | The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of threonine 125 (P-E-TP-P-R). |
Application
Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody.The lane on the right is treated with the antigen-specific peptide.
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | WB: 1:500 - 1:1000. IHC: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
