Application
Western blot analysis of extracts from 293 and Hela cells using Caspase 9 Antibody .| Product Name | Caspase 9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caspase 9Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. |
| Immunogen | Peptide sequence around aa.301~305 (T-S-P-E-D) derived from Caspase 9 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | APAF-3 APAF3 CASPASE-9c ICE-LAP6 MCH6 CASP9 apoptotic protease MCH-6 caspase-9 apoptotic protease activating factor 3 protein phosphatase 1 regulatory subunit 56 ICE-like apoptotic protease 6 caspase 9 apoptosis-related cysteine peptidase |
|---|---|
| Target | Caspase 9 |
| Molecular Weight(MW) | 47, 37, 35 (note) |
| Tissue Specificity | It could both recognize pro-form caspase 9 and caspase 9 subunit p35. |
Application
Western blot analysis of extracts from 293 and Hela cells using Caspase 9 Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
