Application
Immunofluorescence of Caspase-9 in K562 cells with Caspase-9 antibody at 20 ug/mL.Application
Western blot analysis of caspase-9 in HeLa whole cell lysate with Caspase-9 antibody at 1 ug/mL.Application
Immunocytochemistry staining of K562 cells using Caspase-9 antibody at 2 ug/mL.| Product Name | Caspase 9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caspase 9This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] |
| Immunogen | Caspase-9 antibody was raised against a 20 amino acid peptide near the center of human Caspase-9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIPWB |
| WB: 1 ug/ml. ICC: 2 ug/ml. IF: 20 ug/ml: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | APAF-3 MCH6 PPP1R56 CASP9 ICE-LAP6 APAF3 caspase 9 apoptosis-related cysteine peptidase |
|---|---|
| Target | Caspase 9 |
| Tissue Specificity | Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family. |
Application
Immunofluorescence of Caspase-9 in K562 cells with Caspase-9 antibody at 20 ug/mL.
Application
Western blot analysis of caspase-9 in HeLa whole cell lysate with Caspase-9 antibody at 1 ug/mL.
Application
Immunocytochemistry staining of K562 cells using Caspase-9 antibody at 2 ug/mL.| Application Notes | WB: 1 ug/ml. ICC: 2 ug/ml. IF: 20 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
