Application
Western blot analysis of extracts of various cell lines, using CBFB antibody.Application
Immunofluorescence analysis of U2OS cell using CBFB antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded Mouse brain using CBFB antibody at dilution of 1:100 (x400 lens).Application
Immunohistochemistry of paraffin-embedded human stomach cancer using CBFB antibody at dilution of 1:200 (x400 lens).| Product Name | CBFb antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CBFbThe protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human CBFB |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | core-binding factor beta subunit CBFB PEBP2B |
|---|---|
| Target | CBFb |
Application
Western blot analysis of extracts of various cell lines, using CBFB antibody.
Application
Immunofluorescence analysis of U2OS cell using CBFB antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded Mouse brain using CBFB antibody at dilution of 1:100 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded human stomach cancer using CBFB antibody at dilution of 1:200 (x400 lens).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
