Application
ELISA was performed using a serial dilution of CBFb antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,800.Application
ChIP was performed with SKNO-1 nuclear extract and either 4 μl of CBFb antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MYOG, FUT7, OGG1, NFE2 or SPI1.| Product Name | CBFb antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CBFb |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAChIP |
| ChIP/ChIP-seq (4 μl/ChIP), ELISA (1:1,000): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | core-binding factor beta subunit PEBP2B CBFB |
|---|---|
| Target | CBFb |
Application
ELISA was performed using a serial dilution of CBFb antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,800.
Application
ChIP was performed with SKNO-1 nuclear extract and either 4 μl of CBFb antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MYOG, FUT7, OGG1, NFE2 or SPI1.| Application Notes | ChIP/ChIP-seq (4 μl/ChIP), ELISA (1:1,000): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Whole antiserum from rabbit containing 0.05% azide. |
