Mouse monoclonal antibody [4C6] to CCR6This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene. [provided by RefSeq, Jul 2008]
Immunogen
Recombinant human CCR6 (1-46aa) purified from E. coli
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4C6
Host Species
Mouse
Tested Applications
ELISAWB
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
WB analysis of Raji lysate (30ug) using CCR6 antibody at a dilution of 1:500.
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.:
Additional Information
Form
Liquid
Storage Instructions
Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Storage Buffer
Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.