Application
FACS analysis of CD148 expression on U937 cells (shaded histogram). Open histogram indicates the reaction of isotypic control to the cells.Application
IP analysis of biotinylated PBMC with mouse IgG2a (1) or CD148 antibody (2). The immunoprecipitated immunocomplex was resolved on SDS-PAGE and immunoblotted with HRP-Streptavidin.Application
FACS analysis of CD148 expression on Lymphocyte (1), Monocyte (2), and Granulocyte (3).The staining intensity of M086-3 is shown in the horizontal axis with CD11b-FITC staining on the vertical axis.| Product Name | CD148 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [#255] to CD148The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | human peripheral blood mononuclear cells |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | #255 |
| Host Species | Mouse |
| Tested Applications | FACSIP |
| Flow cytometry: 5 μg/mL (final concentration). Immunoprecipitation: 2 μg/200 μL of cell extract from 5x106: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | PTPRJ R-PTP-ETA SCC1 DEP1 protein tyrosine phosphatase receptor type J HPTPeta |
|---|---|
| Target | CD148 |
Application
FACS analysis of CD148 expression on U937 cells (shaded histogram). Open histogram indicates the reaction of isotypic control to the cells.
Application
IP analysis of biotinylated PBMC with mouse IgG2a (1) or CD148 antibody (2). The immunoprecipitated immunocomplex was resolved on SDS-PAGE and immunoblotted with HRP-Streptavidin.
Application
FACS analysis of CD148 expression on Lymphocyte (1), Monocyte (2), and Granulocyte (3).The staining intensity of M086-3 is shown in the horizontal axis with CD11b-FITC staining on the vertical axis.| Application Notes | Flow cytometry: 5 μg/mL (final concentration). Immunoprecipitation: 2 μg/200 μL of cell extract from 5x106: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 1 mg/mL in PBS/50% glycerol, pH 7.2 |
