Application
IHC image using -Detection of CD15 by IHC in human Hodgkins lymphoma| Product Name | CD15 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MMA] to CD15The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. CD15 antibodies recognize the tri saccharide 3 fucosyllactosamine (3Fl) which is present in lacto N fucopentaose III and in the blood group antigen X hapten. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. |
| Immunogen | BALB/C mice were injected with U937 histiocytic cell line. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | MMA |
| Host Species | Mouse |
| Tested Applications | IHCIHC-P |
| IHC-P: 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Synonyms | SSEA1 FUC-TIV FucT-IV FAL 2526 AI451562 ELFT FCT3A Fut4 FUCTIV LeX 14345 CD15 P22083 104230 FUC TIV FucT IV |
|---|---|
| Target | CD15 |
| Tissue Specificity | This antibody reacts with a 220 kD protein. |
| Cellular Localization | Type II membrane protein. Membrane bound form in trans cisternae of Golgi. |
| Light Chain Type | kappa |
Application
IHC image using -Detection of CD15 by IHC in human Hodgkins lymphoma| Positive Control | Tonsil |
|---|---|
| Application Notes | IHC-P: 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: Sodium Azide. |
