| Product Name | CD22 antibody (Functional Grade) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Functional Grade Mouse Monoclonal antibody [IS7] to CD22CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.Entrez Gene: 19q13.1 |
| Immunogen | Reh human cell line |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | IS7 |
| Host Species | Mouse |
| Tested Applications | FACS |
| FACS: Positive Control: lysed whole blood. Note: lyse, wash protocol: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MGC130020 SIGLEC-2 SIGLEC2 BL-CAM BLCAM |
|---|---|
| Target | CD22 |
| Tissue Specificity | The antibody IS7 reacts with CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes. |
| Application Notes | FACS: Positive Control: lysed whole blood. Note: lyse, wash protocol: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. |
