Monoclonal Mouse Antibody to Human Endothelial Cell CD31This antibody reacts with endothelial cells and is specific against a glycoprotein of 100 kD. This antibody stains platelets as well as B cells in the mantle zone, neutrophils and peripheral T cells.
Immunogen
BALB/C mice were injected with the membrane preparation of spleen of a patient with hairy cell leukemia.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
JC/70A
Host Species
Mouse
Tested Applications
IHC-P
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of formalin fixed tissue sections with proteolytic enzymes is recommended before immunostaining.:
Formalin fixed paraffin embedded human tonsil stained with CD31 antibody (cat. # ).
Positive Control
Tonsil
Application Notes
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of formalin fixed tissue sections with proteolytic enzymes is recommended before immunostaining.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C.