Application
Surface staining of human platelets with anti-CD42b: PE .| Product Name | CD42b antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [HIP1] to CD42bGlycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that is linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Several polymorphisms and mutations have been described in this gene, some of which are the cause of Bernard-Soulier syndromes and platelet-type von Willebrand disease. [provided by RefSeq] |
| Immunogen | Tissue/cell preparation (Human). Peripheral blood mononuclear cells of a patient suffering with CLL. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | HIP1 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-F |
| For FACS: Use at an assay dependent dilution. The antibody HIP1 inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets and ristocetin-induced platelet agglutination. For IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
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| Species Reactivity | HumanPrimate |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | P07359 MGC34595 CD42B CD42balpha GP1B GP1BA 177820 BSS CD42b-alpha 2811 CD42b alpha |
|---|---|
| Target | CD42b |
Application
Surface staining of human platelets with anti-CD42b: PE .| Application Notes | For FACS: Use at an assay dependent dilution. The antibody HIP1 inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets and ristocetin-induced platelet agglutination. For IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 15 mM sodium azide |
