Application
IHC-P analysis of human tonsil tissue using CD45RA antibody [F8-11-13].| Product Name | CD45RA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [F8-11-13] to Human CD45RA sampleThe CD45 molecule is comprised of different glycoproteins ranging from 180-240 kD. Expression of CD45 is found on all hemopoietic cells. Detection of the different isoforms can distinguish between different cell forms i.e. naive T cells and memory T cells. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. The functional activity of each CD45 isotype also varies. |
| Immunogen | Human T lymphocytes |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | F8-11-13 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIHC-P |
| For Flow Cytometry: Use at a dilution of 1:50 - 1:100. For IHC-P: use an assay dependent concentration. Optimal dilutions/concentrations should be determined by the researcher. *This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.: |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Q14761 CD45AP PTPRCAP 601577 5790 CD45RA LPAP CD45-AP CD45 AP |
|---|---|
| Target | CD45RA |
| Tissue Specificity | recognises the human CD45RA cell surface antigen, the high molecular weight form of the leucocyte common antigen (LCA). CD45RA is expressed by B lymphocytes and a subset of T lymphocytes. |
| Cellular Localization | Cell membrane |
Application
IHC-P analysis of human tonsil tissue using CD45RA antibody [F8-11-13].| Positive Control | Tonsil |
|---|---|
| Application Notes | For Flow Cytometry: Use at a dilution of 1:50 - 1:100. For IHC-P: use an assay dependent concentration. Optimal dilutions/concentrations should be determined by the researcher. *This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.2, containing 0.09% sodium azide |
