Application
Surface staining of lysed and washed porcine peripheral blood with purified anti-CD47 (detection by anti-mouse IgM: FITC). Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03; ) Panel B, C, D - porcine PBMC sample stained with anti-CD47 Cells in the granulocyte gate were used for analysis.| Product Name | CD47 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 122] to CD47This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq] |
| Immunogen | COS-7 cells transfected with human CD47. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | MEM-122 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FWB |
| FACS: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. WB: Use at a dilution of 1/100. Use in non-reducing conditions. This antibody causes platelet aggregation and ATP release from platelet-rich plasma but not from washed platelets, indicating a complement-dependent reaction. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMonkeyPigPrimate |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 601028 IAP OA3 MER6 CD47 961 Q08722 |
|---|---|
| Target | CD47 |
| Tissue Specificity | Integrin Associated Protein |
Application
Surface staining of lysed and washed porcine peripheral blood with purified anti-CD47 (detection by anti-mouse IgM: FITC). Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03; ) Panel B, C, D - porcine PBMC sample stained with anti-CD47 Cells in the granulocyte gate were used for analysis.| Application Notes | FACS: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. WB: Use at a dilution of 1/100. Use in non-reducing conditions. This antibody causes platelet aggregation and ATP release from platelet-rich plasma but not from washed platelets, indicating a complement-dependent reaction. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4 °C for 3 months. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
