Application
ELISA analysis of control antigen and antigen using CD59 antibody [8D2B8].Application
WB analysis of human CD59 recombinant protein using CD59 antibody [8D2B8].Application
WB analysis of lysates from (1) untransfected and (2) CD59 (a.a. 31-111)-hIgGFc transfected HEK293 cells using CD59 antibody [8D2B8].Application
IHC-P analysis of bladder cancer tissues using CD59 antibody [8D2B8].Application
IHC-P analysis of esophageal cancer tissues using CD59 antibody [8D2B8].Application
ICC/IF analysis of MCF-7 cells using CD59 antibody [8D2B8] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
FACS analysis of HeLa cells using CD59 antibody [8D2B8] (green) or a negative control (red).Application
ICC/IF analysis of HeLa cells using CD59 antibody [8D2B8] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).| Product Name | CD59 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [8D2B8] to CD59This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 31 and 111 of human CD59 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8D2B8 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | EJ16 16.3A5 MIC11 HRF20 MIN1 MSK21 MAC-IP HRF-20 MIRL G344 MACIF EJ30 P18-20 MIN3 MIN2 EL3 |
|---|---|
| Target | CD59 |
| Molecular Weight(MW) | 14.2 kDa (note) |
Application
ELISA analysis of control antigen and antigen using CD59 antibody [8D2B8].
Application
WB analysis of human CD59 recombinant protein using CD59 antibody [8D2B8].
Application
WB analysis of lysates from (1) untransfected and (2) CD59 (a.a. 31-111)-hIgGFc transfected HEK293 cells using CD59 antibody [8D2B8].
Application
IHC-P analysis of bladder cancer tissues using CD59 antibody [8D2B8].
Application
IHC-P analysis of esophageal cancer tissues using CD59 antibody [8D2B8].
Application
ICC/IF analysis of MCF-7 cells using CD59 antibody [8D2B8] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
FACS analysis of HeLa cells using CD59 antibody [8D2B8] (green) or a negative control (red).
Application
ICC/IF analysis of HeLa cells using CD59 antibody [8D2B8] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
