Application
FACS analysis of human peripheral blood lymphocytes using CD59 antibody [MEM-43] (Azide free).| Product Name | CD59 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [MEM-43] to CD59This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | MEM-43 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-FIHC-PIPWB |
| Flow Cytometry: 1/5 - 1/10 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MACIF 1F5 MIN2 MIN3 HRF-20 16.3A5 MSK21 EJ16 HRF20 EL32 CD59 molecule complement regulatory protein G344 MIC11 MIN1 MEM43 MAC-IP MIRL EJ30 p18-20 |
|---|---|
| Target | CD59 |
| Tissue Specificity | This antibody reacts with a PI-linked glycoprotein, Mw 18-20kDa found on all types of leucocytes including platelets. The antigen is expressed on many haemapoietic and non-haemapoietic cells. MEM-43 has been shown to stimulate NK activity. CD59 is expres |
Application
FACS analysis of human peripheral blood lymphocytes using CD59 antibody [MEM-43] (Azide free).| Application Notes | Flow Cytometry: 1/5 - 1/10 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline |
