Application
Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 (MEM-259) FITC .Application
Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).Application
Immunofluorescence staining of CD63 in human primary fibroblasts using anti-CD63 (MEM-259)(green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).Application
Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259 ; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).| Product Name | CD63 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 259] to CD63CD63 is intracellular lysosomal/endosomal/granule protein that is translocated to cell surface upon activation of platelets, endothelial cells, and granulocytes (activation marker). CD63 is also expressed on monocytes/macrophages and endothelium. Its cellular function has not been exactly elucidated. CD63 is thought to be associated with the early stages of melanoma tumor progression (in regulation of motility and adhesion of melanoma cells). |
| Immunogen | Tissue / cell preparation (Human). (T cell line HPB-ALL) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-259 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-PIPWB |
| For FACS: Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils. For IP: Use at an assay dependant concentration. For WB: Use at an assay dependant dilution. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LAMP-3 ME491 MLA1 OMA81H TSPAN30 |
|---|---|
| Target | CD63 |
| Tissue Specificity | The antibody MEM-259 reacts with CD63 (Melanoma 1 antigen), a 40-60 kDa glycoprotein (tetraspans family, TM4SF). CD63 is translocated to the cell surface upon activation of platelets, endothelial cells, and granulocytes (activation marker); it is also expressed on monocytes/macrophages and endothelium. |
Application
Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 (MEM-259) FITC .
Application
Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).
Application
Immunofluorescence staining of CD63 in human primary fibroblasts using anti-CD63 (MEM-259)(green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Application
Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259 ; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).| Application Notes | For FACS: Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils. For IP: Use at an assay dependant concentration. For WB: Use at an assay dependant dilution. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
