Application
CDC2 (phospho Tyr15) antibody detects CDC2 (phospho Tyr15) protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. CDC2 (phospho Tyr15) antibody diluted at 1:500.Application
CDC2 (phospho Tyr15) antibody detects CDK1 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) B. 30 µg HeLa whole cell lysate/extract (2mM double thymidine treatment and recovery for 3.5hr) C. 30 µg HeLa whole cell lysate/extract (2mM double thymidine treatment and recovery for 12hr) 10 % SDS-PAGE CDC2 (phospho Tyr15) antibody dilution: 1:1000Application
Immunoprecipitation of CDC2 (phospho Tyr15) protein from HeLa whole cell extracts using 5 μg of CDC2 (phospho Tyr15) antibody . Western blot analysis was performed using CDC2 (phospho Tyr15) antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
CDC2 (phospho Tyr15) antibody detects CDK1 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) B. 30 µg HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr) 10 % SDS-PAGE CDC2 (phospho Tyr15) antibody dilution: 1:1000| Product Name | CDC2 (phospho Tyr15) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC2 (phospho Tyr15) (cyclin-dependent kinase 1)The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Modification | p-Tyr15 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Zebrafish, Japanese medaka, Drosophila, Xenopus laevis, Pig, Chicken, Sheep, Rhesus Monkey, Chimpanzee, BovineMouse (100%), Zebrafish (100%), Japanese medaka (100%), Drosophila (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDC2 antibody CDC28A antibody DKFZp686L20222 antibody MGC111195 antibody P34CDC2 antibody CDK1 antibody p34 protein kinase antibody cell division protein kinase 1 antibody cell cycle controller CDC2 antibody cell division cycle 2 G1 to S and G2 t |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (5) |
| Cellular Localization | Nucleus |
Application
CDC2 (phospho Tyr15) antibody detects CDC2 (phospho Tyr15) protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. CDC2 (phospho Tyr15) antibody diluted at 1:500.
Application
CDC2 (phospho Tyr15) antibody detects CDK1 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) B. 30 µg HeLa whole cell lysate/extract (2mM double thymidine treatment and recovery for 3.5hr) C. 30 µg HeLa whole cell lysate/extract (2mM double thymidine treatment and recovery for 12hr) 10 % SDS-PAGE CDC2 (phospho Tyr15) antibody dilution: 1:1000
Application
Immunoprecipitation of CDC2 (phospho Tyr15) protein from HeLa whole cell extracts using 5 μg of CDC2 (phospho Tyr15) antibody . Western blot analysis was performed using CDC2 (phospho Tyr15) antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
CDC2 (phospho Tyr15) antibody detects CDK1 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) B. 30 µg HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr) 10 % SDS-PAGE CDC2 (phospho Tyr15) antibody dilution: 1:1000| Positive Control | HeLa (untreated) , HeLa (2mM double thymidine treatment and recovery for 3.5hr) , HeLa (2mM double thymidine treatment and recovery for 12hr) , HeLa (100ng/ml Nocodazole treatment for 24hr) , Rat-2 (untreated) , Rat-2 (100ng/ml Nocodazole treatment for 24 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
