Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using CDC2 antibody at 1:500 dilution.Application
Immunoprecipitation of CDC2 protein from A431 whole cell extracts using 5 μg of CDC2 antibody . Western blot analysis was performed using CDC2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
CDC2 antibody detects CDC2 protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract 10 % SDS-PAGE CDC2 antibody dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE diluted at 1:500| Product Name | CDC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC2 (cyclin-dependent kinase 1)The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CDC2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Japanese medaka, Xenopus laevis, Pig, Chicken, Sheep, Rhesus Monkey, Chimpanzee, BovineRat (97%), Zebrafish (83%), Japanese medaka (83%), Xenopus laevis (88%), Pig (98%), Chicken (93%), Sheep (98%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDC2 antibody CDC28A antibody DKFZp686L20222 antibody MGC111195 antibody P34CDC2 antibody CDK1 antibody p34 protein kinase antibody cell division protein kinase 1 antibody cell cycle controller CDC2 antibody cell division cycle 2 G1 to S and G2 t |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using CDC2 antibody at 1:500 dilution.
Application
Immunoprecipitation of CDC2 protein from A431 whole cell extracts using 5 μg of CDC2 antibody . Western blot analysis was performed using CDC2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
CDC2 antibody detects CDC2 protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract 10 % SDS-PAGE CDC2 antibody dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE diluted at 1:500| Positive Control | A431 , Jurkat , GL261 , NIH-3T3 , BCL-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
