Application
DNA polymerase delta antibody detects POLD1 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 5 % SDS-PAGE DNA polymerase delta antibody dilution: 1:1000Application
DNA polymerase delta antibody detects DNA polymerase delta protein at cytoplasm and nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. DNA polymerase delta antibody diluted at 1:250.Application
Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:3000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA Polymerase delta, catalytic subunit antibody at 1:200 dilution.| Product Name | DNA polymerase delta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNA polymerase delta (polymerase (DNA directed), delta 1, catalytic subunit 125kDa)The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA; MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1, POLD2 (MIM 600815), POLD3 (MIM 611415), and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DNA polymerase delta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, BovineMouse (92%), Bovine (98%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDC2 antibody POLD antibody POLD1 antibody CDC2 homolog antibody DNA-directed polymerase delta 1 antibody DNA polymerase subunit delta p125 antibody DNA polymerase delta catalytic subunit antibody polymerase (DNA directed) delta 1 catalytic subuni |
|---|---|
| Molecular Weight(MW) | 124 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
DNA polymerase delta antibody detects POLD1 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 5 % SDS-PAGE DNA polymerase delta antibody dilution: 1:1000
Application
DNA polymerase delta antibody detects DNA polymerase delta protein at cytoplasm and nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. DNA polymerase delta antibody diluted at 1:250.
Application
Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:3000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA Polymerase delta, catalytic subunit antibody at 1:200 dilution.| Positive Control | Molt-4 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
