Application
Immunohistochemical analysis of paraffin-embedded human testis tissues using anti-CDC25C mouse mAbApplication
Western blot analysis using anti-CDC25C mAb against Hela(1), K562(2), PC-3(3), HEK293(4) and Raw264.7(5) cell lysate.| Product Name | CDC25C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1F12] to CDC25CThis gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 1 and 200 of human CDC25C. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1F12 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| Recommended Starting Dilutions:
ELISA: 1/10000.
WB: 1/500 - 1/2000.
IHC-P: 1/200 - 1/1000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PPP1R60 CDC25 |
|---|---|
| Target | CDC25C |
| Molecular Weight(MW) | 58 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded human testis tissues using anti-CDC25C mouse mAb
Application
Western blot analysis using anti-CDC25C mAb against Hela(1), K562(2), PC-3(3), HEK293(4) and Raw264.7(5) cell lysate.| Application Notes | Recommended Starting Dilutions:
ELISA: 1/10000.
WB: 1/500 - 1/2000.
IHC-P: 1/200 - 1/1000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
