| Product Name | CDK7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MO - 1.1] to CAKCDK7 (CAK) is cdk activating kinase associated with the 34 kDa cyclin H protein, and a36kDa unnamed protein. CDK7 / cyclin H is an integral part of the general transcription factor complex TFIIH; hence is important for activated transcription of polymerase II-dependent promoters and DNA repair. CDK7 is nuclear at all stages of the cell division cycle, except for mitosis indicating that its activity is required for key CDKs operating indistinct cell cycle phases. |
| Immunogen | A recombinant 221 amino acid fragment from the C-terminal of human CAK. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | MO-1.1 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-FIPWB |
| Kinase assay: assy dependentFlow cytometry: assay dependent. Immunofluorescence: assay dependent. Immunoprecipitation (with protein A): use at a concentration of 2μg/mg protein lysate (native and denatured). Western blot: use at a concentration of 1 - 2μg/ml for 2 hours at RT. Immunohistochemistry (frozen sections): assay dependent. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of ~ 40 kDa, which corresponds to the predicted molecular weight of CAK.: |
|
| Species Reactivity | HumanRat |
| Concentration | 0.2mg/ml |
| Gene Synonyms | 601955 p39MO15 1022 CDKN7 P50613 CDK7 CAK1 STK1 |
|---|---|
| Target | CAK1 |
| Cellular Localization | Nuclear and cytoplasmic. |
| Positive Control | HT29 and LS174T cells. |
|---|---|
| Application Notes | Kinase assay: assy dependentFlow cytometry: assay dependent. Immunofluorescence: assay dependent. Immunoprecipitation (with protein A): use at a concentration of 2μg/mg protein lysate (native and denatured). Western blot: use at a concentration of 1 - 2μg/ml for 2 hours at RT. Immunohistochemistry (frozen sections): assay dependent. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of ~ 40 kDa, which corresponds to the predicted molecular weight of CAK.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
