Application
Immunohistochemical detection of CENP-A on paraffin embedded section of a squamous epithelium of human tonsil.Application
This picture shows human metaphase chromosomes detected with as primary antibody and AF488 goat anti-mouse secondary antibody (green). This image was kindly supplied as part of the review submitted by Professor Beth A. Sullivan, Boston UniversityApplication
Western blot analysis of Jurkat (1), Raji (2), PC12 (3), Rat-1 (4), WR19L (5), C2C12 (6), and NIH3T3 (7) cell lysates probed with CENP-A mAbApplication
Hela cells (human), Friend cells (mouse) and calf thymus (Roche). 5 g acid-extracted histones were loaded per lane. Images taken following 5 min exposure. 1:1000 dilution. Lane 1: Friend cells (mouse); Lane 2: Hela cells (human); Lane 3: Calf thymus. This| Product Name | CENPA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3 - 19] to CENPACentromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. |
| Immunogen | Synthetic peptide: PRRRSRKPEAPRRRSPS, corresponding to amino acids 3-19 of Human CENP A. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3-19 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PNeutWB |
| ICC: Use at a concentration of 10 μg/ml. IHC: Use at a concentration of 5 μg/ml. For paraffin embedded sections, heat treatment may be necessary. Microwave sections 2 times for 10 minutes each in citrate buffer (pH 6.5). WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa). Optimal dilutions/concentrations should be determined by the end user. User review: This antibody is great for immunofluorescence on human cells (primary fibroblasts and transformed cell lines such as HeLa, HT1080, A549, H1299). Works well with various fixation conditions, including 1-4% paraformaldehyde (PFA), -20C methanol, 2% PFA followed by -20C methanol, and on unfixed preparations. We routinely use it at a dilution of 1:200-1:400.: |
|
| Species Reactivity | HumanChicken |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | 1058 CENPA 117139 P49450 |
|---|---|
| Target | CENPA |
| Cellular Localization | Nuclear |
Application
Immunohistochemical detection of CENP-A on paraffin embedded section of a squamous epithelium of human tonsil.
Application
This picture shows human metaphase chromosomes detected with as primary antibody and AF488 goat anti-mouse secondary antibody (green). This image was kindly supplied as part of the review submitted by Professor Beth A. Sullivan, Boston University
Application
Western blot analysis of Jurkat (1), Raji (2), PC12 (3), Rat-1 (4), WR19L (5), C2C12 (6), and NIH3T3 (7) cell lysates probed with CENP-A mAb
Application
Hela cells (human), Friend cells (mouse) and calf thymus (Roche). 5 g acid-extracted histones were loaded per lane. Images taken following 5 min exposure. 1:1000 dilution. Lane 1: Friend cells (mouse); Lane 2: Hela cells (human); Lane 3: Calf thymus. This| Positive Control | *BE cells |
|---|---|
| Application Notes | ICC: Use at a concentration of 10 μg/ml. IHC: Use at a concentration of 5 μg/ml. For paraffin embedded sections, heat treatment may be necessary. Microwave sections 2 times for 10 minutes each in citrate buffer (pH 6.5). WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa). Optimal dilutions/concentrations should be determined by the end user. User review: This antibody is great for immunofluorescence on human cells (primary fibroblasts and transformed cell lines such as HeLa, HT1080, A549, H1299). Works well with various fixation conditions, including 1-4% paraformaldehyde (PFA), -20C methanol, 2% PFA followed by -20C methanol, and on unfixed preparations. We routinely use it at a dilution of 1:200-1:400.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None Constituents: 50% Glycerol, PBS. pH 7.2 |
