| Product Name | CENPE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [1H12] to CENP-ECENPE is a 250-300 kDa human centromere-associated kinesin-like motor protein that accumulates in G2 phase. In contrast to other centromere proteins, CENPE is not detected at centromeres during interphase, and first appears at the centromere region of chromosomes during prometaphase. CENPE function is required for the transition from metaphase to anaphase. CENPE is probably one of the motors responsible for mammalian chromosome movement and/or spindle elongation. |
| Immunogen | Recombinant full length CENP-E protein (human) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1H12 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| For ICC/IF: Use at 0.5-1μg/ml: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 1058 CENPA 117139 P49450 CENPE |
|---|---|
| Target | CENP-E |
| Tissue Specificity | Human CENP-E. Not tested on reactivity with rodents or other vertebrates |
| Cellular Localization | Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height. |
| Light Chain Type | kappa |
| Application Notes | For ICC/IF: Use at 0.5-1μg/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. For extended storage, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS + 0.1% sodium azide |
