| Product Name | CFTR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MM13 - 4] to CFTRCFTR (Cystic Fibrosis Transmembrane Conductance Regulator) is composed of two membrane spanning domains (MSD), two nucleotide binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3,5 cyclic monophosphate (cAMP) activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation. |
| Immunogen | The immunogen corresponding to a region within amino acids 1387 and 1480 of human CFTR. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MM13-4 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-P |
| Immunofluorescence: assay dependent. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 1 - 2µg / ml for 60 min at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | CFTR P13569 ABCC7 TNRCFTR MRP7 1080 TNR-CFTR 219700 dJ760C5.1 CF ABC35 TNR CFTR |
|---|---|
| Target | CFTR |
| Cellular Localization | Cell membrane. |
| Positive Control | Pancreas |
|---|---|
| Application Notes | Immunofluorescence: assay dependent. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 1 - 2µg / ml for 60 min at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
