Application
Detection of human Chk1 protein in HeLa whole cell lysate using GeneTex Chk 1 6F5 monoclonal antibodyApplication
Chk1 antibody [6F5] detects Chk1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg A375 whole cell lysate/extract 10 % SDS-PAGE Chk1 antibody [6F5] dilution: 1:500Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 10% SDS PAGE diluted at 1:500| Product Name | Chk1 antibody [6F5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal to human Chk1 [6F5]Chk1 is a kinase involved in the DNA damage checkpoint response, and can phosphorylate Cdc25C. It is hypothesized that Chk1 induces serine 216 phosphorylation of Cdc25C and subsequent binding negatively regulated Cdc25C, thus preventing it from activating Cdc2. |
| Immunogen | Full-length human Chk1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 6F5 |
| Host Species | Mouse |
| Tested Applications | WB |
| For WB: Use at an assay dependent dilution. Predicted molecular weight: 52 kDa. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 603078, 1111, O14757, Chk1, CHEK1 |
|---|---|
| Target | Chk1 |
| Tissue Specificity | Human Chk1 |
| Cellular Localization | Nuclear |
Application
Detection of human Chk1 protein in HeLa whole cell lysate using GeneTex Chk 1 6F5 monoclonal antibody
Application
Chk1 antibody [6F5] detects Chk1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg A375 whole cell lysate/extract 10 % SDS-PAGE Chk1 antibody [6F5] dilution: 1:500
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 10% SDS PAGE diluted at 1:500| Application Notes | For WB: Use at an assay dependent dilution. Predicted molecular weight: 52 kDa. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing no preservatives |
