Application
Application| Product Name | Chk1 antibody [DCS-310] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DCS-310] to Chk1One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic dependent kinase Cdc2, which regulates entry into mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of Cdc2. It is hypothesized that Chk1 induces serine 216 phosphorylation of Cdc25C and subsequent 14-3-3 binding negatively regulated Cdc25C, thus preventing it from activating Cdc2. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | DCS-310 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| IHC-P: Use at a concentration of 1 μg/ml. Heat treatment is neccessary for paraffin embedded sections. Heat in a microwave oven, twice for 10 minutes in citrate buffer, pH 6.5.IP: Use at a concentration of 2 μg per 0.2 ml of cell extract. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa). ECL detection is used. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 603078, 1111, O14757, Chk1, CHEK1 |
|---|---|
| Target | Chk1 |
| Cellular Localization | Nuclear |
Application
Application| Application Notes | IHC-P: Use at a concentration of 1 μg/ml. Heat treatment is neccessary for paraffin embedded sections. Heat in a microwave oven, twice for 10 minutes in citrate buffer, pH 6.5.IP: Use at a concentration of 2 μg per 0.2 ml of cell extract. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa). ECL detection is used. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC - Storage at temperatures lower than -20 °C is not recommended. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.2% BSA and 0.09% sodium azide |
