| Product Name | cK-Ras antibody [234-4.2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [234-4.2] to cK-RasThis gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant fragment, corresponding to C terminal amino acids 54-189 of Rat cK RAS. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 234-4.2 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-PIPWB |
| For IHC-Fr: Use at a concentration of 10 μg/ml. For IHC-P: Use at a concentration of 10 μg/ml. For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 5 μg/ml. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | C-K-RAS , NS , KRAS1 , K-RAS4A , KRAS , KI-RAS , KRAS2 , K-RAS2B , NS3 , K-RAS2A , K-RAS4B , RASK2 |
|---|---|
| Target | cK Ras |
| Tissue Specificity | This antibody reacts with cK Ras and vK Ras, and cross-reacts with cH Ras or cN Ras p21s in immunoblotting. For this reason we recommend a sample concentration step prior to immunoblotting; concentrate to 5 µg/ml with 1% gelatin as a stabilizer. For immunoprecipitation, use 5 µg per sample with 45 µl protein A agarose. |
| Light Chain Type | kappa |
| Positive Control | SW480, Y1 cells, or normal skin tissue |
|---|---|
| Application Notes | For IHC-Fr: Use at a concentration of 10 μg/ml. For IHC-P: Use at a concentration of 10 μg/ml. For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 5 μg/ml. Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5, containing 0.09% sodium azide |
