Application
WB analysis of HEK293 cell lysate using c-Myc antibody [9E10].Application
ELISA analysis of c-myc tagged recombinant protein using c-Myc antibody [9E10].| Product Name | c-Myc antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse monoclonal [9E10] to c-MycMyc (cMyc) is a protooncogene, which is overexpressed in a wide range of human cancers. When it is specifically-mutated, or overexpressed, it increases cell proliferation and functions as an oncogene. Myc gene encodes for a transcription factor that regulates expression of a great number of genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). Myc belongs to Myc family of transcription factors, which also includes N-Myc and L-Myc genes. Myc-family transcription factors contain the bHLH/LZ (basic Helix-Loop-Helix Leucine Zipper) domain. |
| Immunogen | Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of human c-myc |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 9E10 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHC-FIHC-PIPWB |
| For WB: 1:500-1:3000.For FACS: Use at a dilution of neat - 1:10. For IHC-Fr and IHC-P: Use at an assay dependent dilution. For ELISA and WB: Use at a dilution of 1:100 - 1:500. Optimal concetrations/dilutions should be determined by the researcher.*This product does not require protein digestion pre-treatment of paraffin sections prior to staining. **This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | c Myc cMyc |
|---|---|
| Target | c-Myc |
| Tissue Specificity | detects the 62kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. p62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers. |
Application
WB analysis of HEK293 cell lysate using c-Myc antibody [9E10].
Application
ELISA analysis of c-myc tagged recombinant protein using c-Myc antibody [9E10].| Positive Control | Raji , HeLa nuclear |
|---|---|
| Application Notes | For WB: 1:500-1:3000.For FACS: Use at a dilution of neat - 1:10. For IHC-Fr and IHC-P: Use at an assay dependent dilution. For ELISA and WB: Use at a dilution of 1:100 - 1:500. Optimal concetrations/dilutions should be determined by the researcher.*This product does not require protein digestion pre-treatment of paraffin sections prior to staining. **This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4, containing 0.09% sodium azide |
