Application
c-Myc antibody [GT168] detects Myc protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract 10 % SDS-PAGE c-Myc antibody [GT168] dilution: 1:500Application
c-Myc antibody [GT168] antibody detects c-Myc protein by flow cytometry analysis. Sample: NT2D1 cell fixed in 4% paraformaldehyde for 15 min on ice. Black: Unlabelled sample was also used as a control. Red: c-Myc antibody [GT168] antibody dilution: 1:50. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.Application
c-Myc antibody [GT168] detects Myc protein at nucleus on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. c-Myc antibody [GT168] dilution: 1:200.Application
c-Myc antibody [GT168] detects C-MYC protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: c-Myc antibody [GT168] dilution: 1:50Application
Immunoprecipitation of c-Myc protein from HeLa nuclear extracts using 5 μg of c-Myc antibody [GT168] . Western blot analysis was performed using c-Myc antibody [GT168] . EasyBlot anti-Mouse IgG was used as a secondary reagent.| Product Name | c-Myc antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse Monoclonal antibody [GT168] to c-Myc (v-myc myelocytomatosis viral oncogene homolog (avian))The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT168 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MRTL antibody bHLHe39 antibody c-Myc antibody MYC antibody avian myelocytomatosis viral oncogene homolog antibody myc-related translation/localization regulatory factor antibody class E basic helix-loop-helix protein 39 antibody transcription factor |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) |
| Cellular Localization | Nucleus |
Application
c-Myc antibody [GT168] detects Myc protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract 10 % SDS-PAGE c-Myc antibody [GT168] dilution: 1:500
Application
c-Myc antibody [GT168] antibody detects c-Myc protein by flow cytometry analysis. Sample: NT2D1 cell fixed in 4% paraformaldehyde for 15 min on ice. Black: Unlabelled sample was also used as a control. Red: c-Myc antibody [GT168] antibody dilution: 1:50. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.
Application
c-Myc antibody [GT168] detects Myc protein at nucleus on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. c-Myc antibody [GT168] dilution: 1:200.
Application
c-Myc antibody [GT168] detects C-MYC protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: c-Myc antibody [GT168] dilution: 1:50
Application
Immunoprecipitation of c-Myc protein from HeLa nuclear extracts using 5 μg of c-Myc antibody [GT168] . Western blot analysis was performed using c-Myc antibody [GT168] . EasyBlot anti-Mouse IgG was used as a secondary reagent.| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
