Application
Western blot of rat neonatal brain lysate showing specific immunolabeling of the ~62k COBRA1 protein using COBRA1 (NELF-B) antibody [F7E4] .| Product Name | COBRA1 (NELF-B) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [F7E4] to COBRA1 (NELF-B)Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding. |
| Immunogen | Recombinant full length human COBRA1 purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | F7E4 |
| Host Species | Mouse |
| Tested Applications | WB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | DKFZP586B0519 NELFB COBRA1 NELF-B NELF B |
|---|---|
| Target | COBRA1 (NELF-B) |
| Molecular Weight(MW) | 62 (note) |
Application
Western blot of rat neonatal brain lysate showing specific immunolabeling of the ~62k COBRA1 protein using COBRA1 (NELF-B) antibody [F7E4] .| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
