Application
GeneTex anti collagen II antibody ( 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.| Product Name | Collagen II antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Collagen IICollagens are highly conserved throughout evolution and are characterized by an uninterrupted ""Glycine-X-Y"" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein. |
| Immunogen | Collagen Type II from human knee cartilage and bovine nasal cartilage. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | BovineHumanMouseRatSheep |
| Predicted Reactivity | Mouse, Rat, Sheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ANFH COL11A3 COL2A1 AOM SEDC |
|---|---|
| Target | Collagen II |
Application
GeneTex anti collagen II antibody ( 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.| Positive Control | * chondrocyte tissue |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 and 0.01% (w/v) Sodium Azide |
